Technology
3C™ Technology
Cross Contamination Check
Principle
HB 3C™ Technology is PCR technology that determines false positive results that occur due to cross-contamination by synthetic oligomers from the positive control.
3C™ Technology & 3C™ Probe
Factors causing False Positives
Ⅰ
During nucleic acid extraction/purification
Equipment management
Ⅱ
Laboratory contamination by PCR products
Use of UDG enzyme
Ⅲ
PCR equipment
Separation of PCR room
Ⅳ
Cross contamination by positive control
3C™ Technology
Precisely discriminate the possibility of false positive by Cross-contamination of Positive control without affecting target gene’s PCR results
True Positive
3C™ (Cross Contamination Check) Probe does not function due to the absence of the 3C™ Probe binding sequence in the sample.
PCT contamination in sample
HB BestPlex™3C Flu/COVID-19 RT PCR Kit uses 3C™ Probe to check the sample for cross-contamination by the PCT (Positive Control Template).
