Technology

Ⅳ.

3C™ Technology

Check of Cross Contamination

Principle : 3C™

HB 3C™ Technology is,

PCR technology that determines false positive results that occur due to cross-contamination by synthetic oligomers from the positive control.

Factor causing False Positives

in Molecular Diagnostics

Cross contamination during
Nucleic acid extraction/purification

Equipment management

Laboratory contamination
by PCR products

Use of UDG enzyme

PCR equipment

Separation of PCR room

Cross contamination
by positive control

Negative Control

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3C™ Technology

Cross Contamination of Positive Control

Determination of false positives

True Positive
3C_graph1

3C (Cross Contamination Check) Probe does not function due to the absence of the 3C Probe binding sequence in the sample.

PCT contamination in sample

HB BestPlex™

3C Flu/COVID-19 RT PCR Kit uses

3C Probe to check the sample for cross-contamination by the PCT (Positive Control Template).

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