Technology
3C™ Technology
Cross Contamination Check
HB 3C™ Technology is PCR technology that determines false positive results that occur due to cross-contamination by synthetic oligomers from the positive control.
Factors causing False Positives
1.
During nucleic acid extraction/purification
Equipment management
2.
Laboratory contamination by PCR products
Use of UDG enzyme
3.
PCR equipment
Separation of PCR room
4.
Cross contamination by positive control
Precisely discriminate the possibility of false positive by Cross-contamination of Positive control without affecting target gene’s PCR results
True Positive
3C™ (Cross Contamination Check) Probe does not function due to the absence of the 3C™ Probe binding sequence in the sample.
PCT contamination in sample
HB BestPlex™3C Flu/COVID-19 RT PCR Kit uses 3C™ Probe to check the sample for cross-contamination by the PCT (Positive Control Template).
