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하임바이오텍
miRNA detection

miRNA (microRNA)

Single-stranded RNA without protein synthesis information consisting of 18-25 ribonucleic acid bases.
(Early) Biomarkers for diagnosis of cancer and various diseases. (Blood / Tissue)
Central regulator of gene expression in cells. (regulates 30-92% of total genes)
Biomarker for (early) diagnosis of infectious diseases caused by viruses, etc.
Cell homeostasis is maintained by engaging in all life phenomena including cell proliferation and death, tumor formation and morphogenesis, hematopoietic process and immune cell development and differentiation.
In the spotlight as a next-generation molecular diagnostic tool that solves the limitations of existing molecular diagnostics.

Regulation of gene expression
after transcription by miRNA

Inhibits protein synthesis and induces RNA degradation by binding complementarily to target miRNA

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miRNA_img

Heim Biotek's miRNA detection technology

Korea's first microRNA (hereinafter referred to as
microRNA) analysis source technology developed
and possessed (world's three major technologies)
Differentiation from overseas competitors' technologies

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Competitive technology (Agilent and Applied Biosystems) Heim Biotek's technology
Analysis process RT-qPCR RT-qPCR
Simultaneous multiple analysis impossible possible
Electrophoretic analysis Single: Inaccurate (Agilent) / Multiplex: Impossible exact
Sequencing inaccuracy exact

Characteristics of Heim Biotek's
miRNA detection technology

  • High sensitivity
  • High specificity
  • High reactivity
  • High Competitiveness
  • System
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RNA Ct value Melting
1 ug 13.17 80.50
100 ng 16.62 80.50
10 ng 20.22 80.50
1 ug 24.20 80.50
100 pg 28.14 80.50
10 pg 31.40 80.50
1 pg 36.63 80.50
NTC None None
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Amplification of hsa-miR-106b-5p by HB Multi Assay (System l, SYBR Green).

hsa-let-7e-5p

Competitor product

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Own product

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Competitor product

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Own product

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Competitiveness of clinical diagnostic products

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Competitive products
(based on Qiagen/ABI)
Heim Biotek
SystemⅠ SystemⅡ
Analysis process Real-Time PCR RT / Real-Time PCR RT / Real-Time PCR
Simultaneous
multiple analysis
Impossible - Possible
Real-Time PCR SYBR Green (Qiagen) TaqMan-Probe SYBR Green TaqMan-Probe SYBR Green TaqMan-Probe
Gel electrophoresis
analysis
Single : Inaccuracy / Multiplex : Impossible Accuracy Accuracy
Sequencing
(DNA Sequencing)
Inaccuracy Accuracy Accuracy

System

SystemⅠ

Using poly A tailing technology, from one total RNA sampleCreate cDNA library, use SYBR Green or TaqMan-Probe. System to detect/analyze multiple miRNAs

SystemⅡ

Specifically, only target miRNA is produced as cDNA, SYBR Green or System to detect/analyze target miRNA using TaqMan-Probe
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Heim Biotek Co., Ltd. : Pangyo Silicon Park A-201 35, Pangyo-ro 255beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Company Registration Number : 135-86-48279
Tel : +82-1522-7026
Fax : +82-031-548-2135
E-mail : info@heimbiotek.com
Copyright ⓒ 2020 Heim Biotek All rights reserved.

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