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SNP detection

SNP (Single Nucleotide Polymorphism)

Genetic variation showing a difference in one base (A, T, G, C) in the DNA sequence is called Single Nucleotide polymorphism (SNP).
When an allele is present with a frequency of 5% or more, it is called common polymorphism, and when it is 1 to 5%, it is classified as rare polymorphism.

SNP analysis using specific
bi-directional extension technology (SBDE)

By using the specific bi-directional extension technology, the original technology of Heim Biotek, it is possible to accurately distinguish homozygote and heterozygote simultaneously with one experiment.

Existing method
(eg. Allele Specific-PCR)

snp_img

HB SBDE-PCR

snp_img

Sensitivity improvement using
Switching-SBDE PCR technology

The cross-reaction and low yield, which are the disadvantages of the existing AS-PCR, have been compensated,
and even a small amount of sample has high specificity and sensitivity.

Advantage of HB Switching AS-PCR technology based on T790M detection

[Comparing with General AS-PCR]

Switching PCR
sens_gray_box_img
Condition
95 ℃ 15 min
95 ℃ 15 sec 20 Cycle
65 ℃ 30 sec
72 ℃ 30 sec
95 ℃ 15 sec 40 Cycle
71 ℃ 30 sec
72 ℃ 30 sec
Switching AS-PCR result
>1/8% & 1/10% mutant typing available at 95% level
General PCR
sens_gray_box_img
Condition
95 ℃ 15 min
95 ℃ 15 sec 60 Cycle
71 ℃ 30 sec
72 ℃ 30 sec
General AS-PCR results
>1/8% & 1/10% mutant typing impossible at 95% level
logoImg
HeimBiotek, Inc. : Pangyo Silicon Park A-201 35, Pangyo-ro 255beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea
Company Registration Number : 135-86-48279
Tel : +82-1522-7026
Fax : +82-031-548-2135
E-mail : info@heimbiotek.com
Copyright ⓒ 2020 Heim Biotek All rights reserved.

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