By using the specific bi-directional extension technology, the original technology of Heim Biotek, it is possible to accurately distinguish homozygote and heterozygote simultaneously with one experiment.
Existing method
(eg. Allele Specific-PCR)
HB SBDE-PCR
The cross-reaction and low yield, which are the disadvantages of the existing AS-PCR, have been compensated,
and even a small amount of sample has high specificity and sensitivity.
Advantage of HB Switching AS-PCR technology based on T790M detection
[Comparing with General AS-PCR]
| Condition | ||
|---|---|---|
| 95 ℃ | 15 min | |
| 95 ℃ | 15 sec | 20 Cycle |
| 65 ℃ | 30 sec | |
| 72 ℃ | 30 sec | |
| 95 ℃ | 15 sec | 40 Cycle |
| 71 ℃ | 30 sec | |
| 72 ℃ | 30 sec | |
| Condition | ||
|---|---|---|
| 95 ℃ | 15 min | |
| 95 ℃ | 15 sec | 60 Cycle |
| 71 ℃ | 30 sec | |
| 72 ℃ | 30 sec | |
